College of Life Science and Technology, Beijing Advanced Innovation Center for Soft Matter Science and Engineering, Beijing University of Chemical Technology, Beijing, 100029, China
| Received 05 Jan 2025 |
Accepted 21 Feb 2025 |
Published 26 Feb 2025 |
Throughout thousands of years, the yeast Saccharomyces cerevisiae has been acting as a cell factory in food production. Lately, it has further functioned as a platform cell factory for the production of a multitude of different compounds, spanning from high-volume fuels to high-value pharmaceuticals. The past decade has witnessed the fact that the innovative tools in synthetic biology have driven the rapid progress of the yeast cell factory, enabling us to edit the genetic systems of organisms efficiently and "reprogram" elements or systems such as genes, circuits, pathways, and networks of organisms. Here, we will offer a brief review that highlights the most recent significant advances and perspectives regarding the innovative tools in yeast synthetic biology. These tools encompass genome editing tools, computational tools, adaptive laboratory evolution, and the standardization of biological DNA parts, with the intention of providing a practical guide for the implementation of novel, effective, and efficient development of the customized yeast cell factory.
